| | |
Species Expression
|
Inhibition of Growth
|
|---|
|
Cell Type
|
N
|
44 kD
|
49/53 kD
|
Yes/No (#)
|
|---|
|
Normal, primary cultures
|
133
|
+/-
|
++++
|
Y(2/2)
|
|
Normal breast tissue*
|
5
|
+
|
++
|
NT
|
|
Mammary cell line
|
16
|
+ (11/16)
|
-
|
Y (12/12)
|
|
Breast tumor lysate
|
25
|
+ (17/25)
|
-
|
NT
|
|
Non-Mammary cell lines
|
8
|
+ (3/8) **
|
-
|
N (0/8)
|
-
Analysis of Mammastatin expression by immunoblot: Lysatesof fresh tissue, primary cultures and cell lines were analyzed by 7G6.6,anti-mammastatin, immunoblot. N = number of individual samples repeated at least3 times. (++++) intense expression, (-) no expression, NT = not treated.
-
Inhibition Assays: Cells were plated in growth media at 104cells/ml and treated with 10 ng/ml purified Mammastatin or control. Culturescounted by Coulter Counter after six days culture.
-
Carcinoma cells in which mammastatin was detected: MCF-7,ZR-75-1, T47D, Sk Br 3, ZR-75-30, BT 475, UACC 893, MDA-MB-361, Sum 44, Sum 52,Sum 102 (Sum lines from S. Either, Uof Mich), Hep G2, OvCar, BxPC3, andA253.
-
Carcinoma cells in which mammastatin was not detected:MDA-MB-231, BT-20, MDA-MB-435, HBL-100, Du 4475, UM 17B (squamous line from T.Corbett Uof Mich), SK Mel 31, 293T, HT 1080. * Two additional normaltissue lysates from breast cancer patients showed no Mammastatin expression.** Similar size proteins (between 40 and 55 kD) were identified inBxPc3, HepG2 and OvCar, A253 cells but these cell lines did not producedinhibitory activity.
