Table 1 Oligonucleotide primers used for PTT and SSCP analyses

PTT

BRCA1 exon 11

Br5'-S: 5'-GCTGCTTGTGAATTTTCTGAG-3'

Br5'-A: 5'-GCCTGCAGTGATATTAACTGTCTG-3'

Br3'-S: 5'-GAAGAAAGTGAACTTGATG-3'

Br3'-A: 5'-TAAGTTTGAATCCATGCTTTG-3'

BRCA2 exon 10

B10-S: 5'-TTTGGAAAAACATCAGGGAATT-3'

B10-A: 5'-AAACACAGAAGGAATCGTCATC-3'

BRCA2 exon 11

B11-1S: 5'-CATTCTTCTGTGAAAAGAAGCTG-3'

B11-1A: 5'-TGGTTTGAATTAAAATCCTGC-3'

B11-2S: 5'-TACATGAACAAATGGGCAGGAC-3'

B11-2A: 5'-TCCAGTACCAACTGGGACAC-3'

B11-3S: 5'-GATCAGAAACCAGAAGAATTGC-3'

B11-3A: 5'-TTGGGATATTAAATGTTCTGGAGTA-3'

B11-4S: 5'-TCACCTTGTGATGTTAGTTTG-3'

B11-4A: 5'-GTTAGCATACCAAGTCTACTG-3'

SSCP

BRCA1 exons 2, 3, 5-10, 12-24

Reported by Friedman et al [17]

BRCA2 exons 2-9, 12, 13, 15, 16, 19-27

Reported by Friedman et al [11]

BRCA2 exon 14

B14-S: 5'-GTGTACTAGTCAATAAAC-3'

B14-A: 5'-CATCACACAAATTGTCAT-3'

BRCA2 exon 18

B18-S: 5'-GAATTCTAGAGTCACACTTCCT-3'

B18-A: 5'-ACTGATTTTTACCAAGAGTGCA-3'