Relationship between HER-2 and p21WAF1/CIP1 localisation. Scatter plots according to HER-2 status and the subcellular localisation of p21WAF1/CIP1 measured as an intensity distribution score (IDS). HER-2 staining was analysed as described in Materials and methods. (a)Distribution of nuclear p21WAF1/CIP1 IDS and medians according to HER-2-negative and HER-2-positive breast cancers, with patient numbers shown. (b) Cytoplasmic p21WAF1/CIP1 IDS and medians according to HER-2 status, and numbers of patients. Of the 67 patients, nuclear p21WAF1/CIP1 was not evaluable in four patients, or in the cytoplasm in six cases. P values indicate significance.