Figure 4

Multiple myoepithelial cell layer disruptions and overlying estrogen receptor (ER)-negative cell clusters in ductal carcinoma in situ. (a) A paraffin section immunostained for ER (brown) and smooth muscle actin (SMA) (red), 200×; (b) a higher magnification of (a), 400×; (c) a paraffin section immunostained for ER (brown) and SMA (red), 200×; (d) a higher magnification of (c), 400×. Myoepithelial cell layer disruptions and ER-negative cell clusters are identified with arrows.