Table 3 Antibodies used for immunohistochemistry
From: aHIF but not HIF-1α transcript is a poor prognostic marker in human breast cancer
Antibody | Target | Source | Dilution | Antigen-retrieval method | Staining |
|---|---|---|---|---|---|
6F11 | Oestrogen receptor | Tébu (Le Perray en Yvelines, France) | 1:40 | Pressure cooker, 3 min, citrate buffer pH 7.3 | Nuclear |
1α6 | Progesterone receptor | Tébu (Le Perray en Yvelines, France) | 1:30 | Pressure cooker, 3 min, citrate buffer pH 7.3 | Nuclear |
DA 485 | HER2 | Dako (Trappes, France) | 1:1500 | Water bath, 40 min, citrate buffer pH 6.0 | Membrane |
DO7 | Wild-type and mutated p53 | Dako (Trappes, France) | 1:200 | Pressure cooker, 3 min, citrate buffer pH 7.3 | Nuclear |