Table 2 Oligonucleotide sequences used for RT–PCR, and amplicon sizes
From: aHIF but not HIF-1α transcript is a poor prognostic marker in human breast cancer
Oligonucleotide | Direction | Sequence | Product size |
|---|---|---|---|
HIF-1α | Forward | GTC GGA CAG CCT CAC CAA ACA GAG C | 486 bp and 359 bp if exon 14 missing |
Reverse | GTT AAC TTG ATC CAA AGC TCT GAG | ||
aHIF | Forward | TTT GTG TTT GAG CAT TTT AAT AGG C | 279 bp |
Reverse | CCA GGC CCC TTT GAT CAG CTT | ||
β2-microglobulin | Forward | CAT CCA GCG TAC TCC AAA GA | 165 bp |
Reverse | GAC AAG TCT GAA TGC TCC AC |