Figure 2

Immunostaining pattern of ME cells in ductal carcinoma in situ (case 2). (a) H&E staining; (b) immunostaining for SMA; (c-f) double immunostaining of SMA with maspin, CK5, CK14, and CK17, respectively, and the entire ME cell layer is negative for these antibodies in some ducts and is also negative for SM-MHC, WT-1, CD10, and calponin (not shown).