Table 3 Primers for RT-PCR
From: β class II tubulin predominates in normal and tumor breast tissues
β-Tubulin gene | Description | Sequence (5'→ 3') | T m (°C) | Amplicon length (base pairs) |
|---|---|---|---|---|
Class I | Forward | CCCCATACATACCTTGAGGCGA | 59.8 | 289 |
Reverse | GCCAAAAGGACCTGAGCGAA | 59.1 | ||
Class II | Forward | ACGGGTTAGGGAAAGCGGA | 59.6 | 241 |
Reverse | TTCCGACACAAACGTTTATGTGA | 56.7 | ||
Class III | Forward | ATGCGGGAGATCGTGCACAT | 60.6 | 238 |
Reverse | CCCCTGAGCGGACACTGT | 60.0 | ||
Class IVa | Forward | TCTCCGCCGCATCTTCCA | 59.8 | 272 |
Reverse | GCTCTGGGGACATAATTTCCTCCT | 60.0 | ||
Class IVb | Forward | GCTGTTTGTCTACTTCCTCCTGCT | 60.4 | 344 |
Reverse | CAGTTGTTCCCAGCACCACTCT | 60.8 | ||
Class V | Forward | CGGGGAGGAAGCTTTTGAGGAT | 60.5 | 244 |
Reverse | CTGGGTAGAACCCGCAATTCTCT | 60.2 | ||
Class VI | Forward | AGTTGTGTTGGGCTCACACCA | 60.8 | 133 |