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Figure 6 | Breast Cancer Research

Figure 6

From: Host microenvironment in breast cancer development: Epithelial-cell–stromal-cell interactions and steroid hormone action in normal and cancerous mammary gland

Figure 6

Estrogen-responsiveness in MCF-7 wild-type and LM-specific α6 integrin transfectants. Wild-type MCF-7 cells were plated (50,000 cells per well) on 24-well plates on either Col I or LM (a) and α6a and α6 transfectants were plated on Col I (b) in serum-free medium (SFM). The cells were treated with ICI 182,780 (200 nM) for 24 hours, followed by indicated treatments (17β-estradiol [E2] 20 nM, IGF-1 25 ng/ml) for 24 hours, labeled with 3H-thymidine for 3 hours and assayed for 3H-thymidine incorporation into DNA. Col I, collagen type 1; E2, 17β-estradiol; ICI, ICI 182,780; IGF-1, insulin-like growth factor I; LM, laminin.

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