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Table 1 Primers used in representational difference analysis (RDA) and different PCR screening experiments

From: A candidate metastasis-associated DNA marker for ductal mammary carcinoma

Primer/product size Primer sequence (forward) Primer sequence (reverse)
RDA representation R24, 5'-agcactctccagcctctcaccgca-3' R12, 5'-gatctgcggtga-3'
RDA first-round hybridization J24, 5'-accgacgtcgactatccatgaaca-3' J12, 5'-gatctgttcatg-3'
RDA second-round hybridization N24, 5'-aggcaactgtgctatccgagggaa-3' N12, 5'-gatcttccctcg-3'
RDA third-round hybridization Same as first round (J24) Same as first round (J12)
PTEN (556 bp) 5'-ctcagattgccttataatagtc-3' 5'-tcatgttactgctacgtaaac-3'
MADS-IX (180 bp) 5'-aggtaggtagagtaacaggtttgttt-3' 5'-gatctgttctcccttttctttagctt-3'
FISH fragment (2 kb) 5'-actatgtatgtgagcatctgtttgc-3' 5'-tgcactcttcacttgagtaaacttg-3'
β-Actin (295 bp) 5'-tcacccacactgtgcccatctacga-3' 5'-cgacgtagcacagcttctcctta-3'
  1. FISH, fluorescence in situ hybridization.