Figure 5

PCR screening of MADS-IX and PTEN on DNA samples from four tumor cell lines and matched normal DNA. Results (first row) show that MADS-IX is present in all the tumor cell lines (HCC-1806, HCC-1143, HCC-1428 and HCC-1937) especially the HCC-1937 cell line, which has loss of a region encompassing the PTEN gene. Screening of PTEN in these four cell lines (second row) show its presence in the HCC-1806, HCC-1143 and HCC-1428 tumor cell lines but, as expected, it is missing in HCC-1937. β-Actin was used as an internal control.