Figure 4

PCR screening of MADS-IX on normal cell DNA, primary cell DNA and metastatic cell DNA samples of five patients. PCR results show the target DNA band (180 bp) missing in patients 1 and 3. While MADS-IX showed a reduction in the intensity (about one-half) of the PCR band (heterozygous loss) in the primary tumors (P) of patients 1 and 3, it is completely missing (homozygous loss) in their metastatic (M) cell DNA samples but present in normal cell DNA (N). β-Actin was used as an internal control.