Figure 2
From: A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland
Expression of T-Ag in the mammary glands of transgenic mice harbouring one to two copies (line SV40-2) or about 10 copies (line SV40-13) of the BLG-tsA58 transgene. (a) Protein extracts (20 μg) were prepared from day 11 lactation mammary tissue from three age-matched sisters from each line and a nontransgenic control mouse. The extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, electroblotted onto nitrocellulose and probed with an anti-T-Ag antibody, DP-02. The nonspecific band is also seen in the control sample. (b) Morphological comparison of haematoxylin and eosin stained sections of virgin and 11-day lactation mammary glands from transgenic line SV40-2 (top) and a nontransgenic control (bottom). Histologically, the transgenic glands were indistinguishable from control glands. Scale bar 100 μm.