The effect of estrogen deprivation and estrogen treatment on the growth of BRCA1 antisense and control cells. (A) The mean cell number of BRCA1 pooled population antisense infected cells (filled circle) and vector-only infected cells (white circle) deprived of estrogen for up to 20 days after plating 2.5×106 cells per 100-mm dish. Cells received a pretreatment of 5 days of estrogen deprivation before plating. (B) The percentage decrease or fold increase of BG-1 antisense (AS) or NEO clones and parental cells plated at 106 cells per 60-mm dish after deprivation of estrogen (solid bars) or treatment with 10-8 mol/l estrogen (spotted bars) for 8 days. Percentage decrease or fold increase was calculated on the basis of the change in cell number between days 5 and 8 after treatment or nontreatment with 10-8 mol/l estrogen. Cells were pretreated with 5 days of estrogen deprivation before plating.