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Figure 3 | Breast Cancer Research

Figure 3

From: The role of bisphosphonates in breast cancer: Direct effects of bisphosphonates on breast cancer cells

Figure 3

(a) Attenuation by broad-spectrum caspase inhibitor z-VAD-fmk (zVAD) of the effects of pamidronate (APD) on cell viability in MDA-MB-231 cells. Cells were plated in 96-well plates (1 × 103 cells/well) and treated with 50 μmol/l zVAD 1 h before addition of 30 μmol/l APD for 3 days. On days 1, 2 and 3, cell viability was quantitated using MTS dye reduction assay. Results are shown as mean ± SD. ** P < 0.0001 versus APD + zVAD treatment. (b) Effects of zVAD on APD-induced DNA fragmentation in MDA-MB-231. Cells were plated in 6-well plates at a density of 1 × 106/well and treated with 50 μmol/l of zVAD for 1 h before addition of 30 μmol/l APD for 4 days without renewal of medium. Cotreatment with zVAD significantly reduced APD-induced DNA fragmentation. * P < 0.0005 versus APD treatment alone.

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