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Figure 6 | Breast Cancer Research

Figure 6

From: P2Y2receptor activation by nucleotides released from highly metastatic breast cancer cells increases tumor growth and invasion via crosstalk with endothelial cells

Figure 6

Inhibition of P2Y 2 R reduced breast cancer cell growth and metastasis in an in vivo mouse model. Athymic nude mice were divided into two groups and injected subcutaneously with empty vector-transfected MDA-MB-231 (MDA-MB-231-EV; n = 10) or P2Y2R-shRNA-transfected MDA-MB-231 (MDA-MB-231-P2Y2R-shRNA; n = 10) (5 × 106 cells/100 μl of serum-free medium). (A) MDA-MB-231-EV-injected or MDA-MB-231-P2Y2R-shRNA-injected animals were sacrificed at day 60, and the tumors were extracted. Body weights (B) and tumor volumes (C) were measured every 3 days during tumor development (*P <0.05, **P <0.01 compared to the MDA-MB-231-EV-injected group). (D) Incidence of lung metastasis was examined in MDA-MB-231-EV- or MDA-MB-231-P2Y2R-shRNA-injected mice, and representative HE-stained sections are shown (*P < 0.05, compared to the MDA-MB-231-EV-injected group). (E) Lung tissue sections were stained with anti-vimentin, ICAM-1, VCAM-1, CD31 and VEGF antibodies.

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