Figure 4From: P2Y2receptor activation by nucleotides released from highly metastatic breast cancer cells increases tumor growth and invasion via crosstalk with endothelial cellsMatrix metalloproteinase (MMP) activity and vascular endothelial growthe factor (VEGF) production in MDA-MB-231 cells was regulated by P2Y 2 R activation mediated by ATP or uridine 5′-triphosphate (UTP). (A) MDA-MB-231 were treated with the indicated doses of ATP or UTP for 6 h. MMP gelatinase activities were measured in conditioned media (CM) as described in Methods. Control- or P2Y2R-shRNA-transfected MDA-MB-231 were treated with 10 μM of ATP or UTP. After 6 h, MMP gelatinase activities were determined in the CM and quantified (MMP-9; 92 KDa). Significance compared to the control, **P <0.01; significance compared to ATP or UTP, #P <0.05, ##P <0.01. (B) Control- or P2Y2R-shRNA-transfected MDA-MB-231 were treated with 10 μM ATP or UTP for 24 h, and the concentration of VEGF from the media was determined using a quantitative VEGF ELISA. Significance compared to the control, **P <0.01; significance compared to ATP or UTP, ##P <0.01.Back to article page