Ectopic microRNA-18a expression modified gene expression profiles of MB231RN-LM cells in response to CoCl
treatment (200 μM for 4 hours). (A) Venn diagrams of cobalt(II) chloride (CoCl2)–regulated genes (fold change ≥ 1.5, CoCl2 () vs untreated, false discovery rate = 0.1) in LM-miR-18a and LM-GFP cells. GFP, Green fluorescent protein; miR-18a, MicroRNA-18a. (B) Signaling pathways differentially affected by CoCl2 in LM-miR-18a and LM-GFP cells. Enriched signaling pathways of CoCl2-regualted genes were identified using Ingenuity Pathway Analysis software. AMPK, 5′ adenosine monophosphate-activated protein kinase; GM-CSF, Granulocyte-macrophage colony-stimulating factor; HGF, Hepatocyte growth factor; HIF1A, Hypoxia-inducible factor 1α; ILK, Integrin-linked kinase; mTOR, Mammalian target of rapamycin.