Effects of ectopic microRNA-18a expression on gene expression and cell growth in MB231RN-LM cells. (A) Expression levels of microRNA-18a (miR-18a) in cells transduced with lentiviral vector expressing miR-18a green fluorescent protein (GFP) (LM-miR-18a) and or control cells transduced with lentiviral vector expressing GFP only (LM-GFP). (B) Effect of miR-18a on expression levels of predicted target mRNAs. mRNA levels were examined by quantitative PCR (qPCR) and normalized to ACTB. The expression levels of all the presented genes were significantly reduced by miR-18a expression (n = 3, P < 0.05 by Student’s t-test). (C) Effect of miR-18a on RNA-induced silencing complex (RISC) binding of predicted target mRNAs. RISC binding was examined by AGO2 immunoprecipitation (AGO2-IP) followed by RNA purification and qPCR, presented as fold change enrichment (AGO2-IP vs control immunoglobulin G immunoprecipitation). *P < 0.05 by Student’s t-test (fold enrichment of AGO2-IP in LM-miR-18a cells vs in control cells). (D) Growth curves of LM-miR-18a and LM-GFP cells. All results are presented as mean ± SD (n = 3). *P < 0.05 by Kruskal-Wallis analysis of variance followed by Dunn’s multiple-comparisons test).