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Figure 6 | Breast Cancer Research

Figure 6

From: Ubiquitin-conjugating enzyme complex Uev1A-Ubc13 promotes breast cancer metastasis through nuclear factor-кB mediated matrix metalloproteinase-1 gene regulation

Figure 6

Matrix metalloproteinase (MMP)1 is a downstream effector for ubiquitin conjugating enzyme variant (Uev)1A-induced metastasis. (A) The mRNA levels of MMP1, MMP9 and UEV1A in MMP1 or MMP9 knocked-down MDA-MB-231 cells as determined by qRT-PCR. (B) Quantitative analysis of cell invasive ability in Matrigel-coated transwells. MDA-MB-231 cells depleted of MMP1 or MMP9 were subject to the transwell assay and at least five random fields were counted under a light-microscope at 200× magnification. (C) Quantitative analysis of cell invasive ability in Matrigel-coated transwells. MDA-MB-231 cells overexpressing MMP1 were subject to the transwell assay and at least five random fields were counted under a light-microscope at 200× magnification. (D) Ectopic expression of MMP1 restored the MMP1 transcript level in two independent UEV1-depleted cell lines as determined by qRT-PCR. (E) Ectopic expression of MMP1 restored the MMP1 protein level in two independent UEV1-depleted cell lines as determined by western blot. (F) Quantitative analysis of cell invasive ability in Matrigel-coated transwells. At least five random fields were counted under a light-microscope at 200× magnification.

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