Figure 2

Expressions of TAAs and pathological responses to PPV. After completion of eight vaccinations in combination with two cycles of eribulin mesylate (1.4 mg/m²/week for two weeks followed by one week intermission), the lung metastasis of a SD case with mrTNBC (case 18 in Table S2) was resected at three months after the first vaccination. The TAA expression and T cell infiltration in the resected lung tissue were examined by immunohistochemistry. a, b) Among the four TAAs, that is, SART2, PSA, EGF-R and LCK, two TAAs were expressed in the lung tumor. a) EGF-R (X200); b) SART2 (X200). c, d) Peritumoral infiltration of T lymphocytes was confirmed in the lung tumor. c) CD4⁺ T lymphocytes (X200); d) CD8+ T lymphocytes (X200). EGF-R, epidermal growth factor receptor; LCK, lymphocyte specific protein tyrosine kinase; mrTNBC, metastatic recurrent triple negative breast cancer; PPV, personalized peptide vaccination; PSA, prostate specific antigen; SART2, squamous cell carcinoma antigen recognized by T-cells 2; SD, stable disease; TAA, tumor associated antigens.