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Figure 3 | Breast Cancer Research

Figure 3

From: Transforming growth factor beta receptor type III is a tumor promoter in mesenchymal-stem like triple negative breast cancer

Figure 3

TβRIII-KD in MSL cell lines does not affect cell proliferation. A) Live cell count proliferation assay for SUM159, MDA-MB-231 and MDA-MB-157 controls versus TβRIII-KD 72 hours post treatment with TGF-β1 and TGF-β2 ligands; graph bars represent the mean of six replicates with SEM error bars (ns = not significant; *P <0.01, ***P ≤0.0005 for a two-tailed Student’s t-test). B) Thymidine incorporation proliferation assay for SUM159, MDA-MB-231 and MDA-MB-157 controls versus TβRIII-KD in the presence or absence of TGF-β1 and TGF-β2 ligands; graph bars represent the mean of six replicates with SEM error bars (***P ≤0.0004 for a two-tailed Student’s t-test). C) Immunoblot analysis of cleaved-caspase 3 and PARP protein expression using lysates harvested from SUM159, MDA-MB-231 and MDA-MB-157 stably expressing control and TβRIII-KD. Results are representative of three independent experiments. MSL, mesenchymal stem-like; SEM, standard error of the mean; TGF-β, transforming growth factor beta; TβRIII-KD, type III transforming growth factor-beta receptor knockdown.

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