Figure 3

Notch4 and Nicastrin knockdown reduce epithelial to mesenchymal transition (EMT) genes expression. (A) qRT-PCR showing Notch4 and Nicastrin knockdown effect on EMT as well as Notch-responsive genes. microRNA (mRNA) levels are normalised to GAPDH. (n = 3 bars represent standard deviation (SD) ^*P <0.05.) (B) Representative images showing E-cadherin expression in MCF7 after control, Notch4 or Nicastrin knockdown. (C) Representative western blot confirming specificity for small interfering RNA (siRNA) targeting Notch4. IQGAP1 was chosen as EMT representative protein (n = 3, bars represent SD ^*P <0.05). (D) Representative western blot confirming Nicastrin siRNA effect on Notch receptors and IQGAP1 (n = 3, bars represent SD ^*P <0.05).