Table 1 Primer sequences for HER2 chimeric antigen receptor construct
From: Construction and evaluation of a novel humanized HER2-specific chimeric receptor
Gene names | Primer names | Primer sequences (5′-3′) |
|---|---|---|
CD8a leader | AF | gcggaattcatggccttaccagtgaccgccttgctcctgccg (EcoRI) |
Her2scFv | BF | tgctccacgccgccaggccgagatctgacattgtgctgacccaaac |
BR | tgacgagacggtgactgaggttcc | |
CD8a hinge | CF | cctcagtcaccgtctcgtcaaccacgacgccagcgccgcg |
CR | atcacaggcgaagtccagccccc | |
CD28TM + ICD | DF | gggggctggacttcgcctgtgatttttgggtgctggtggtggttg |
DR | ggagcgataggctgcgaagtcgc | |
CD3ζ | EF | gcgacttcgcagcctatcgctccagagtgaagttcagcaggagc |
| Â | ER | gcggtcgacttagcgagggggcagggcctg (SaI1) |