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Figure 1 | Breast Cancer Research

Figure 1

From: Construction and evaluation of a novel humanized HER2-specific chimeric receptor

Figure 1

Schematic diagram of the chA21-28z chimeric antigen receptor construct and chimeric gene products generated by overlap extension PCR. (A) Diagram of the HER2-specific chimeric antigen receptor (CAR), consisting of a humanized chA21 single-chain variable fragment (scFv) linked to CD28 and CD3z signaling moieties. (B) Agarose gel electrophoresis of PCR products. Lane 1, PEE14-chA21 plasmid; lane 2, chA21 scFv. (C) Lane 1, A21 scFv; lane 2, CD8a leader + chA21 scFv; lane 3, CD8a hinge; lane 4, CD28TM + ICD; lane 5, CD8a hinge + CD28 (TM + ICD); lane 6, CD3z; lane 7, CD8a hinge + CD28 (TM + ICD) + CD3z. (D) Lane 1, chA21-28z CAR:CD8a leader + chA21 scFv + CD8a hinge + CD28 (TM + ICD) + CD3z; lane 2, pSin lentiviral vector; lane 3, pSin-chA21-28z CAR plasmid.

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