Figure 3

CXCR7 promotes breast cancer metastasis. Lungs were removed from mice used in the experiment presented in Figure 2 and inflated with Bouin fixative and number of metastatic nodules on the lungs was counted with the aid of a dissecting microscope. (A) H&E (2.5×) staining of metastatic nodules in the lungs of mice bearing 4T1 Vec or 4T1 sh-CXCR7 (insets: 20× magnification of the areas selected by rectangles) (B) bar showing the number of metastatic lung nodules and (C) H&E (2.5X) staining of metastatic nodules in the lungs of mice bearing 4T1.2 tumors treated with either DMSO or CXCR7 inhibitor (CCX771) or STAT3 inhibitor (S31-201) insets: 5× magnification and (D) bar showing the number of metastatic lung nodules. Scale bars, 0.01 mm. **p < 0.01, ***p < 0.001 versus control.