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Figure 5 | Breast Cancer Research

Figure 5

From: The mechanistic target for rapamycin pathway is related to the phosphorylation score for estrogen receptor-α in human breast tumors in vivo

Figure 5

ERα is co-immunoprecipitated with mTOR from MCF7 cells. MCF7 cells were grown in 5% CM (lane 1) or MCF7 were serum starved and estrogen depleted and then treated with (lane 3) and without E2 (lane 2) for 60 minutess. Cells were harvested, crosslinked with DSP and immunoprecipitated with an isotype-matched but irrelevant antibody (IgG) or an antibody specific for mTOR (sc-1549-R). Aliquots of immunoprecipitated complexes were subjected to Western blot analysis with antibodies to ERα or mTOR, as shown. Levels of ERα and mTOR in input lysates are shown in the two lower panels. A representative experiment is shown from a total of three independent experiments. CM, complete medium; DSP, dithiobis(succinimidylpropionate); E2, estradiol; ERα, estrogen receptor α; IgG, immunoglobulin G; mTOR, mechanistic target of rapamycin,

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