Figure 6
Cetuximab combined with lapatinib induces a cooperative antitumor effect in JIMT-1 tumor xenografts. (A) Seven days after orthotopic injection of JIMT-1 cells, mice were randomized (10 per group) to receive cetuximab, lapatinib, or both (see Materials and methods for details). The control group is constituted by animals treated with DMSO vehicle. The one-way ANOVA test was used to compare tumor sizes among treatment groups at the median survival of the control group. Tumors were significantly smaller in mice treated with the combination than in mice treated with a single agent (P < 0.1 × 10-2). (B) Mouse survival was evaluated up to day 49. (C) Western blot analysis of total lysates from JIMT-1 tumor specimens of six mice killed on day 25. Tumors derived from each treatment group were pooled during lysis to obtain a single specimen. The relative optical density of phospho-protein levels normalized to the actin level is shown.