Figure 4From: Epidermal growth factor-receptor activation modulates Src-dependent resistance to lapatinib in breast cancer modelsSrc contributes to lapatinib resistance in breast cancer cells by interacting with EGFR rather than with HER2. (A) Western blot analysis of total cell lysates from MDA-MB-361, SKBR-3, MDA-MB-361-LR, and JIMT-1 human breast cancer cell lines transfected with an Src-specific siRNA (50 nM) and treated or not with lapatinib (1 μM). The siRNA-negative control consists of nontargeting sequences. (B) Immunoprecipitation using anti-HER2, anti-Src, and anti-EGFR antibodies and blotting with anti-EGFR, -HER2, -HER3, and -Src antibodies of MDA-MB-361, SKBR-3, MDA-MB-361-LR, and JIMT-1 human breast cancer cells grown in complete medium. Total lysate, not immunoprecipitated, from BT474 cells was used as positive control. (C) Western blot analysis of total cell lysates from MDA-MB-361, SKBR-3, MDA-MB-361LR, and JIMT-1 human breast cancer cell lines grown in complete medium. The relative optical density of phospho-protein levels normalized to the actin level is shown.Back to article page