A secondary screen of putative negative regulators of TRAIL-induced apoptosis. Four siRNAs corresponding to 16 genes were selected for additional analysis of TRAIL-induced caspase-3/7 activation in (A) MB231, (B) MB468, (C) T47D, and (D) SKBR3. Cells were siRNA transfected, as for the primary screen, and 48 hours later were treated with 1,000 ng/ml TRAIL for 1 hour before assessment of caspase-3/7 activation. The dashed line in each panel indicates the 2-standard deviation fold-increase in TRAIL-induced caspase-3/7 activation compared with TRAIL-treated cells transfected with negative control siRNA (siNeg). The vertical line joins the siRNAs for each gene. Genes are ranked in descending order based on the median value for each set of four siRNAs per gene in the MB231 cell line. Data for the control siRNAs, siNeg, siCASP8, and siFLIP are included for reference.