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Figure 4 | Breast Cancer Research

Figure 4

From: Identification of novel molecular regulators of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in breast cancer cells by RNAi screening

Figure 4

Network analysis of putative negative regulators of TRAIL-induced apoptosis. (A) A gene-interaction network generated by Ingenuity Pathway Analysis of the 150 genes for which three or more siRNAs induced increased TRAIL-induced activation of caspase-3/7 levels. The blue circles indicate those genes with seven or more interactions, and the red rectangles, genes for which three or more siRNAs caused both an increase in TRAIL-induced activation of caspase-3/7 levels and decreased viability in response to TRAIL. Legend shows the symbols indicated by the Ingenuity Pathway Analysis software. (B) Assays of (i) caspase-8, (ii) caspase-3/7 activation, and (iii) cell viability in the absence (empty bars) and presence (black bars) of TRAIL for four siRNAs (A-D) corresponding to the genes shown. Average data (±1 standard deviation) for negative control siRNA (siNeg)-transfected cells is shown in each graph. The grey dashed line indicates the relevant 1 standard deviation value for each assay, and the red dots indicate those siRNAs inducing fold-changes greater than 1 standard deviation.

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