Rapamycin response signature. A. Heatmap of rapamycin response signature gene expression of training set samples with 18 control samples on the left and 5 rapamycin treated samples on the right. Each row is a probe set in the signature. Red indicates up-regulation and blue indicates down-regulation of the gene. B. LOOCV from the Connectivity Map training set samples. On the y-axis, 0 = predicted as untreated; 1 = predicted as treated. Control samples are in blue, and rapamycin treated samples in red. Note that only one control sample was misclassified. C. Heatmap of predicted rapamycin response of 1,401 human breast tumors with a color scaled from red to blue indicating a high to low predicted sensitivity. Each column represents an individual tumor sample, grouped by intrinsic subtypes. D. The percent of samples with predicted rapamycin sensitivity of >0.5 for each intrinsic subtype. The background color represents the overall sensitivity of each subtype at the same scale used in 1C. LOOCV, leave-one-out cross-validation.