Figure 3

PIK3CA sequence variations. A. This is a table of PIK3CA exon mutations in patient and corresponding xenograft samples. The exon number, mRNA position and allele change, and protein position and residue change for each mutation are indicated. B. A sequencing image of patient and corresponding xenograft tumors of the SUTI097 and SUTI110 at the mutated sites. MDA-MB-231 cell line is shown as a normal control. C. Table of PIK3CA intron SNPs that flank sequenced exons in patient and corresponding xenograft samples. Note the conservation of sequence variations between primary tumors and their xenografts, and also between different xenograft passages, before and after rapamycin treatment. SNPs, single nucleotide polymorphisms.