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Figure 3 | Breast Cancer Research

Figure 3

From: Engagement of immune effector cells by trastuzumab induces HER2/ERBB2 downregulation in cancer cells through STAT1 activation

Figure 3

HER2 downregulation in cancer cells by the CM from cancer cells co-cultured with PBMCs in the presence of trastuzumab. Both BT474 (A, C) and SKOV-3 cells (B, D) were cultured for 48 h with 50% CM collected from the co-cultures of four treatment conditions: cancer cell only, cancer cells treated with trastuzumab, cancer cell co-cultured with PBMCs, and cancer cell co-cultured with PBMCs in the presence of trastuzumab. (A-B) WB detection of HER2 level; (C-D) HER2 mRNA levels detected by qPCR, **P <0.01. CM, conditioned media; HER2, human epidermal growth factor receptor 2; PBMC, peripheral blood mononuclear cell; WB, Western blotting.

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