RIP2 knockdown decreases migration and invasion in triple-negative breast cancer ( TNBC) cell lines. (A) Western blot showing relative protein expressions of RIP2 knockdown (si-RIP2 #J and si-RIP2 #D) and pcDNA4 Omni-tagged RIP2 in three TBNC cell lines. (B) Representative scratch/wound healing assay images for MDA-MB-231 cells. (C) Quantification of scratch/wound healing assay results for three triple-negative cell lines and error bars represent standard error of the mean (SEM). Data represent results from three separate experiments. Statistics per Student t-test as indicated. Scale bar = 100 μM. (D) Representative Matrigel™ invasion assays from MDA-MB-231 cells at 16 hours. (E) Quantification of Matrigel™ invasion assay results for three triple-negative cell lines with error bars representing SEM. Columns represent results from triplicates repeated in three separate experiments. Statistics per Student t-test.