Figure 5

Staining controls for different phenotypic markers used. (a) The top three rows are confocal immunofluorescent images of normal donor peripheral blood mononuclear cells (PBMCs), MCF-7 and a mixture of normal donor PBMCs and MCF-7 stained with cytokeratin (CK) antibody (green), CD45 antibody (red), epithelial cell adhesion molecule (EpCAM) (white) and 4′,6-diamidino-2-phenylindole (DAPI; blue). The bottom two rows of images show healthy donor PBMCs and MDA-MB-231 stained with the same antibody dye conjugates and the CK antibody replaced by pan-CK versions. (b) Confocal immunofluorescent images show healthy donor PBMCs, MCF-7 and MDA-MB-231 stained with DAPI (blue); anti-CK7, anti-CK8, anti-CK18 and anti-CK19 antibody (green); antivimentin (red); and anti–epidermal growth factor receptor (EGFR; white).