Table 1 Oligonucleotide primers used for site-directed mutagenesis
Name | Oligonucleotide (5′ to 3′) |
|---|---|
C286A Forward | GCT TTG ATT CTT GCA GTT GCC ATT GCA GTC AAC AGT CG |
C286A Reverse | CGA CTG TTG ACT GCA ATG GCA ACT GCA AGA ATC AAA GC |
C286S Forward | GCT TTG ATT CTT GCA GTT TCC ATT GCA GTC AAC AGT CG |
C286S Reverse | CGA CTG TTG ACT GCA ATG GAA ACT GCA AGA ATC AAA GC |
C295A Forward | CAG TCG AAG AAG GGC TGG GCA GAA GAA AAA GC |
C295A Reverse | GCT TTT TCT TCT GCC CAG CCC TTC TTC GAC TG |