Figure 9

Greater inhibition of MCF-7 cell growth is apparent when AZD8055 is used in combination with tamoxifen or oestrogen deprivation. Seven days growth of oestrogen dependent MCF-7 cells was compared in standard control medium (black squares), in the presence of 10^-7 M tamoxifen (grey square) or oestrogen-deprived medium (X cell medium) (white squares) with a concentration range of AZD8055 (0 to 100 nM). Triplicate wells of cells were counted by Coulter Counter and the results expressed as a percentage of the untreated control cells. Combination with 10 nM AZD8055 significantly increased growth inhibition in the presence of tamoxifen or oestrogen-deprived medium by 66% and 56%, respectively, versus anti-hormone alone. *P <0.05, **P <0.01, ***P <0.001 versus appropriate anti-hormone control in the absence of AZD8055 (0). Results shown are means of three independent experiments. Statistical analysis was performed with ANOVA and post hoc test. ANOVA, analysis of variance.