Figure 6

AZD8055 does not significantly affect ER regulated gene expression in MCF7-X and TamR cells. RNA was extracted from MCF7-X (A) and TamR (B) after 72 hours treatment with AZD8055 (0 to 100 nM) and gene expression analysed by semi-quantitative PCR. The image shows an ethidium bromide stained 2% agarose gel with the upper band showing cyclin D1, pS2 c-myc, bcl2 (only expressed in MCF7-X) and amphiregulin (only expressed in TamR) and the β-actin PCR product (lower band). The bar charts show actin-normalised values for the ER regulated genes which are not significantly affected by AZD8055. Data are representative of two independent experiments. Accompanying immunocytochemical images confirm that 72 hours treatment with a concentration range of AZD8055 (0 to 100 nM) does not significantly affect pS2 or total ER protein expression in MCF7-X or TamR cells (C). Images shown (x20) are representative of three independent experiments.