Figure 3
Effect of AZD8055 on proliferation and viability in TamR and MCF7-X cells. Immunocytochemical evaluation of MIB1 proliferation marker (Ki67) in TamR (A) and MCF7-X cells (B) treated for three days with AZD8055 (0 to 100 nM). Multiple fields of view (x20) were assessed for% cells expressing no/equivocal MIB1 staining. Results are from three independent experiments. * P <0.05 versus untreated control (ANOVA with post-hoc test). Sytox green impermeable nuclear stain was used to measure live cell count in TamR and MCF7-X cells in a viability cell assay before and after three days treatment with AZD8066 (C). After three days in the presence of 25 nM AZD8055, TamR live cell number fell below the pre-treatment count indicating some cell death with this agent. Live cell count (total minus dead cells) was a mean of eight replicates in three independent experiments. *P <0.05, ***P <0.001 for three days treatment with AZD8055 versus appropriate day 0 control ANOVA with post-hoc test. ANOVA, analysis of variance.