Comparison of RAD001 and AZD8055 effect on cell growth and mTORC1/2 signalling pathways in TamR and MCF7-X cells. TamR (black squares) and MCF7-X cells (white squares) were grown for seven days in the presence of RAD001 (0 to 1,000 nM) (A) or AZD8055 (B) (0 to 100 nM) and cell number determined by Coulter Counting. The effect of AZD8055 on seven days growth of tamoxifen-resistant T47D-tamR cells similarly determined is shown in (C). Results expressed as% control are the mean (+/- SEM) of three to five independent experiments. *P <0.05 versus appropriate cell line control (0), **P <0.01 versus appropriate cell line control (0), ***P <0.001 versus appropriate cell line control (0). Western blot of 70% confluent TamR and MCF7-X cells treated for one hour with RAD001 or AZD8055 (0 to 100 nM) (D). Blots were probed with phospho- and total antibodies for mTORC1 (rapamycin sensitive) and mTORC2 (rapamycin insensitive) signalling pathways. Blot shown is representative of at least two independent experiments.