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Figure 2 | Breast Cancer Research

Figure 2

From: Inducible and coupled expression of the polyomavirus middle T antigen and Cre recombinase in transgenic mice: an in vivo model for synthetic viability in mammary tumour progression

Figure 2

PyV mT and Cre recombinase are expressed at all stages of tumourigenesis. (A) Immunohistochemical detection of PyV mT (middle row) and Cre recombinase (bottom row) in ductal structures at the indicated stages of mammary tumour progression in rtTA/MIC mice, contrasted by normal mammary gland histology from control (an un-induced rtTA/MIC) and wildtype mice. The corresponding H&E-stained sections are shown for comparison (top row). (Scale bar: 100 μm). (B) Immunoblot analysis of protein lysates from rtTA/MIC mammary glands (mg), mammary tumours (tum) and adjacent mammary glands (amg) from animals sacrificed at palpation or at end-stage (“late onset” refers to palpation after 16 days of induction) as indicated using antibodies directed to E-cadherin (epithelial content control), PyV mT, Cre recombinase and α-tubulin (loading control). Controls include (from left to right) mammary glands from two un-induced rtTA/MIC mice, one induced MIC mouse and one induced rtTA mouse. Positive controls for PyV mT and Cre recombinase expression were mammary tumours from MMTV-PyV mT (PyV mT) and MMTV-NIC (NIC) animals, respectively; arrowheads indicate specific bands for these proteins.

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Breast Cancer Research

ISSN: 1465-542X

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