Figure 1

Induction of anti-huHER2 antibody response by human cancer cell vaccines with different levels of HER2 membrane expression. Nontransgenic mice received one cycle of vaccination (cells and recombinant interleukin 12) as reported in Methods. The x-axis represents the HER2 expression level (cytofluorometric evaluation by mean fluorescence intensity (MFI) measured in arbitrary units) of cells used for vaccinations, and the y-axis represents the corresponding level of anti-huHER2 antibodies elicited by vaccinations. The dashed line shows the enzyme-linked immunosorbent assay detection sensitivity limit. Data shown are the mean and SEM from five mice per group. huHER2, human HER2 gene.