Figure 3

Unsupervised hierarchical clustering of tumor samples and corresponding expression of PI3K and/or MAPK pathway proteins. Heat map representing unsupervised hierarchical clustering of tumor samples and corresponding expression of downstream activated proteins in the PI3K and/or MAPK pathways from patients for whom the status of all five proteins were known (n = 350). Patients are represented horizontally. Phosphorylated proteins are indicated vertically. Red, high/any expression of phosphorylated protein; green, no/low expression of phosphorylated protein (dichotomization was performed according to Akaike’s information criteria). In addition the presence (red) or absence (green) of different clinico-pathological factors is shown. ERK, extracellular signal-regulated kinase; HER2, human epidermal growth factor receptor 2; MAPK, mitogen-activated protein kinase; mTOR, mammalian target of rapamycine; PI3K, phosphatidylinositol-3-kinase; p, phosphorylated.