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Figure 2 | Breast Cancer Research

Figure 2

From: New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells

Figure 2

Analysis of parity-identified mammary epithelial cells (PI-MECs) during first and second pregnancies. Mammary glands from WAP-Cre; Rosa-lsl-YFP mice were collected at the following stages: 7 days (A) and 14 days (B) of the first pregnancy, 3 days of lactation (C), involuted (>6 weeks after weaning) (D), and 7 days (E) and 14 days (F) of the second pregnancy. Cryosections were analyzed for YFP expression (green) and counterstained with rhodamine-conjugated phalloidin (red). Representative confocal microscope images were captured with 20× (upper panels) and 63× (lower panels) objective lenses. Bar is 50 μm. White arrowhead indicates a PI-MEC (YFPpos cell that survived involution), and the yellow arrows indicate collapsed YFP bodies seen often in involuted sections. (G) Quantification of relative mRNA levels in mammary epithelial organoid isolates. Gene expression is normalized to hypoxanthine-guanine phosphoribosyltransferase (HPRT) expression, and error bars indicate standard deviation of three individual mice at each time point.

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