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Figure 1 | Breast Cancer Research

Figure 1

From: New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells

Figure 1

Labeling of parity-identified mammary epithelial cells (PI-MECs). In WAP-Cre;Rosa26-lsl-YFP double transgenic mice, yellow fluorescent protein (YFP) is not expressed (white cell) until the whey acidic protein (WAP) promoter is induced (by pregnancy). When activation of the WAP promoter results in the expression of sufficient levels of the Cre recombinase, the loxP sites (triangles) will be recombined and the sequence in between will be excised (dotted line), bringing the YFP gene under control of the constitutively active Rosa26 promoter (green cell, WAP on). Upon cell division, daughter cells will remain YFP-positive even though WAP is not expressed (green cell, WAP off) as a result of the genetic deletion of the stop sequence.

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