PR and AP-1 interaction clinical significance. PR and p-c-Jun colocalization in tumor samples. (A) Nuclear p-c-Jun and PR levels were evaluated by IF and scored as described in Results. Protein colocalization was visualized as nuclear yellow dots, indicated by white arrows. Shown are examples of tumors showing 0 to +3 colocalization scores. Nuclei were stained with DAPI (blue). (B) Kaplan-Meier survival analysis correlating levels of p-c-Jun and PR colocalization with overall patient survival. IF, Immunofluorescence; PR, progesterone receptor.