Figure 5
From: GPR30 as an initiator of tamoxifen resistance in hormone-dependent breast cancer
Distribution and expression of GPR30 in MCF-7 and TAM-R cells. The GPR30 subcellular location was detected using immunofluorescent staining. (A) Cells were stained with DAPI, GPR30-GFP alone or a combination (Merge). GPR30 mRNA and protein expressions in MCF-7 and TAM-R cells were quantified by qPCR (B) and western blot (C). Fold changes of GPR30 in total protein and membrane-enriched protein fractions of MCF-7 and TAM-R cells were normalized to β-actin (C). Each experiment was repeated at least three times. Results are expressed as means ± SD. *P <0.05 versus MCF-7 cells. DAPI, 4′, 6-diamidino-2-phenylindole; GPR30, G-protein coupled receptor 30; TAM-R, tamoxifen resistant.