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Table 3 Primers used in QRT-PCR

From: Direct repression of MYB by ZEB1 suppresses proliferation and epithelial gene expression during epithelial-to-mesenchymal transition of breast cancer cells

Gene

Forward primer sequence

Reverse primer sequence

L32

GATCTTGATGCCCAACATTGGTTATG

GCACTTCCAGCTCCTTGACG

Snail1

CCAGACCCACTCAGATGTCAAGAA

GGCAGAGGACACAGAACCAGAAAA

Snail2

CCCAATGGCCTCTCTCCTCTTT

CATCGCAGTGCAGCTGCTTATGTTT

Twist1

CTAGAGACTCTGGAGCTGGATAACTAAAAA

CGACCTCTTGAGAATGCATGCATGAAAAA

Zeb1

GTTACCAGGGAGGAGCAGTGAAA

GACAGCAGTGTCTTGTTGTTGTAGAAA

MYB

CAGGAAGGTTATCTGCAGGAGTCTTCAAAA

CTATAGGCGGAGCCTGAGCAAAA

E-cadherin

GCCCTGCCAATCCCGATGAAA

GGGGTCAGTATCAGCCGCT

Vimentin

GCTTCAGAGAGAGGAAGCCGAAAA

CCGTGAGGTCAGGCTTGGAAA

ERα

GGAGCACCCAGGGAAGCTACTGTTT

GATCTCCACCATGCCCTCTACACATTTT

MKI67

GTAGGTGAGGGCAAAGGCACGAAA

CTTCCGCTTTGCAGGTTGCTTAAA

Primers for ChIP

AGCAGGTGGGAATTCGTTCC

CCAACGTCCGGATACATTTC

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Breast Cancer Research

ISSN: 1465-542X

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