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Figure 1 | Breast Cancer Research

Figure 1

From: Direct repression of MYB by ZEB1 suppresses proliferation and epithelial gene expression during epithelial-to-mesenchymal transition of breast cancer cells

Figure 1

Manipulation of MYB expression influences epithelial/mesenchymal gene expression and clonal morphology. (A) Stable knockdown of MYB in LA cells by shRNA led to mesenchymal gene-expression changes (i) Analysis of MYB expression reduction (notation on graph bar indicates level of fold reduction; n = 3, error bars represent SEM, *, significance set at P < 0.05, Student paired t test) and (ii) further validation of MYB knockdown and analysis of EMT markers CDH1 and VIM with Western blot. (B) Colony morphology was significantly altered in MYB-overexpressing MDA-MB-231 cells. (i) MYB overexpression validated by Western blotting; (ii) examples of the three different colony phenotypes ranging from more-epithelial/compacted (phenotype 1) to most-mesenchymal/scattered (phenotype 3), and representative images of colonies seen in vector control- or MYB-transfected MDA-MB-231 cells, and (iii) increased epithelial (phenotype 1) and decreased mesenchymal (phenotypes 2, 3) seen after MYB transfection of MDA-MB-231 cells (n = 2; ANOVA. **P < 0.005; ****P < 0.0001).

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Breast Cancer Research

ISSN: 1465-542X

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